New technique to isolate Cercospora coffeicola Berkeley & Cooke, etiologic agent of cercospora leaf spot of coffee tree
Keywords:
Coffea arabica, isolation, pipette techniqueAbstract
The stain brown eye is an endemic disease in Brazil. There are few published studies on this disease because of the difficulty of isolation and production of the fungus Cercospora coffeicola. This work aims to publicize this isolation technique developed and used in UFLA called Pipette Technique. Through this already over 70 isolates were obtained for future studies of the etiologic agent of this important disease.References
CUSTÓDIO, A. A. P., POZZA, E. A.; CUSTÓDIO, A. A. P.; SOUZA, P. E.; LIMA, L. A.; LIMA, L. M. Intensidade da ferrugem e da cercosporiose em cafeeiro quanto à face de exposição das plantas. Coffee Science, Lavras, v. 5, n. 3, p. 214-228, set./dez. 2010
LIMA , . L. M.; POZZA, . E. A.; SILVA SANTOS , F. Relationship between Incidence of Brown Eye Spot of Coffee Cherries and the Chemical Composition of Coffee Beans. Journal of Phytopathology, v. 160: p. 209–211, Abril. 2012.
POZZA, E. A.; CARVALHO, L. V.; CHALFOUN, S. M. Sintomas e injúrias causadas por doenças em cafeeiro. In: GUIMARÃES, R. J.; MENDES, A. N. G.; BALIZA, D. P. (Ed.). Semiologia do cafeeiro: sintomas de desordens nutricionais, fitossanitárias e fisiológicas. Lavras: UFLA, 2010. p. 68-106.
SOARES, D. J, Esporulação e germinação in vitro de conídios de Cercospora coffeicola. 2003. p. 31. Dissertação (Mestrado em Fitopatologia) - Universidade federal de Viçosa, Viçosa, 2003.
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